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. 2015 Nov 1;142(21):3734–3745. doi: 10.1242/dev.125328

Fig. 9.

Fig. 9.

Osteogenic differentiation is compromised in Wnt1-Cre;Erk2fl/fl mandibles. (A-D) Active caspase 3 (A,B; green) and Ki67 (C,D; green) immunostaining of control and Wnt1-Cre;Erk2fl/fl mandibles. Arrowheads indicate apoptotic cells. (E,F) Sox9 in situ hybridization of control and Wnt1-Cre;Erk2fl/fl embryos. (G) Quantification from C,D of proliferating cells in three different areas of the mandible: Meckel's cartilage, adjacent mesenchyme, and the osteogenic front (OF). (H-K) Sp7 immunostaining and type 1 collagen (Col1a1) in situ hybridization of control and Wnt1-Cre;Erk2fl/fl mandibles. Arrowhead indicates weak expression of Col1a1. Dashed lines delineate the Meckel's cartilage. n=3 for each analysis. Scale bars: 200 µm.