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. 2015 Nov 1;142(21):3769–3780. doi: 10.1242/dev.127969

Fig. 7.

Fig. 7.

Mislocalized PMCs in miR-31 KD embryos do not express VegfR10. Whole-mount RNA in situ hybridization (WMISH) against VegfR10 was performed in miR-31 KD and control gastrulae. L, lateral; V, ventral. (A) VegfR10 was most strongly expressed in the PMCs located close to the vegetal pole of miR-31 KD embryos. (B) Mislocalized 1D5-immunopositive PMCs in miR-31 KD embryos do not express VegfR10 (arrows). (C) The percentage of embryos with normal PMC patterning was significantly reduced in miR-31 KD gastrulae compared with the control. The percentage of embryos in which mislocalized PMCs did not express VegfR10 was significantly increased in miR-31 KD gastrulae as compared with the control (32.7% 1D5+ VegfR10−). Cochran-Mantel-Haenszel test; error bars indicate s.e.m. Scale bars: 50 µm.