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. 2015 Nov 14;12:185. doi: 10.1186/s12985-015-0380-7

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© Li et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Characterization of recombinant PRRSV expression of the pIL-4 gene in Marc-145 cells. a IFA detection of pIL-4 and N expression in Marc-145 cells infected with parental virus or recombinant virus. Original magnification 200×. b Western blot detection of pIL-4 and N protein in cell lysates of Marc-145 cells infected with recombinant virus and parental virus. c Growth curves of the recombinant and the parental virus in Marc-145 cells. d Kinetics of pIL-4 accumulation in the supernatants of Marc-145 cells infected with the recombinant virus vs. parental virus or mock control. Data are represented as the mean of three independent experiments