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. 2015 Nov 14;10:227. doi: 10.1186/s13014-015-0534-1

Fig. 5.

Fig. 5

IL-6 knocked down CD133+ cells showed lower expression of anti-apoptosis associated molecules. a Western blot analysis of apoptosis associated proteins. CD133+ cells of A549IL-6si/sc and H157IL-6si/sc pairs were irradiated with 6 Gy gamma rays. Two days after irradiation, cell extracts were obtained and expression of Bcl-2 and Mcl-1 were examined by Western blot analyses. b IF staining of Bcl-2 and Mcl-1. CD133+ cells of A549IL-6si/sc and H157IL-6si/sc pairs were plated in chamber slides and irradiated with 6 Gy gamma rays. Two days after irradiation, IF staining was performed using antibodies of Bcl-2 and Mcl-1. *p < 0.05, **p < 0.01