Table 1.
Gene, putative function, CAZy family, induction substrates and significant signal peptide predictions (SignalP 4.0 Server) of major carbohydrate active enzymes transcripts are shown
| Gene | Function | CAZy | Induction substrates | SignalP D score |
|---|---|---|---|---|
| Cellulase | ||||
| AO090701000185 | Endoglucanase A | GH-12 | O-CHE (↓), O-X3G4R (↓), SOP (↓), TYR (↓), O-MHE (↓) | 0.604 |
| AO090001000348 | Cellobiohydrolase C (CelC) | GH-7 | O-CHE (↑), O-X3G4R (↑), O-BGHEXA (↑), O-AHP (↑), SOP (↑), TYR (↑), O-MHE (↑) | 0.661 |
| AO090038000439 | Cellobiohydrolase | GH-6 | O-CHE (↑), O-GMH (↑), O-XPE (↑), SOP (↑) | 0.126a |
| AO090001000266 | β-glucosidase | GH-3 | O-CHE (↓), O-X3G4R (↓), SOP (↓), TYR (↓) | 0.696 |
| AO090001000544 | β-glucosidase | GH-3 | O-MHE (↑) | 0.678 |
| AO090701000274 | β-glucosidase | GH-3 | TYR (↑) | 0.865 |
| AO090023000056 | Polysacch. monooxygenase | GH-61b | O-CHE (↑), O-X3G4R (↑), SOP (↑), TYR (↑), O-MHE (↑) | 0.898 |
| AO090103000087 | Polysacch. monooxygenase | GH-61b, CBM-1 | O-CHE (↓), SOP (↓) | 0.737 |
| AO090012000090 | Polysacch. monooxygenase | GH-61b | SOP (↓) | 0.653 |
| AO090102000058 | Cellobiose dehydrogenase | AA-3 | O-CHE (↑), O-X3G4R (↑), SOP (↑), TYR (↑), O-MHE (↑) | 0.819 |
| AO090003000346 | Aldonolactonase | - | O-CHE (↑), O-X3G4R (↑), O-BGHEXA (↑), O-AHP (↑), SOP (↑) | 0.838 |
| Hemicellulase | ||||
| Xylan | ||||
| AO090026000103 | endo-1,4-β-xylanase | GH-11 | O-CHE (↓), SOP (↓), O-MHE (↓) | 0.874 |
| AO090005000698 | β-xylosidase (XylB) | GH-43 | O-X3G4R (↑), O-XPE (↑) | 0.106 |
| AO090003000239 | xylosidase/arabinosidase or endo-1,4-β-xylanase D | GH-43 | O-CHE (↑), O-X3G4R (↑), O-AHP (↑), O-GM3 (↑), SOP (↑), TYR (↑), O-MHE (↑) | 0.829 |
| AO090001000207 | Feruloyl esterase (FAEA) | - | O-CHE (↑), O-BGHEXA (↑), SOP (↑) | 0.748 |
| AO090020000508 | Acetyl xylan esterase | CE-3 | O-BGHEXA (↑), O-GMH (↑) | 0.449 |
| AO090701000315 | Acetyl esterase | CE-16 | O-CHE (↑), O-X3G4R (↑), O-BGHEXA (↑), O-GMH (↑), O-XPE (↑), O-AHP (↑), O-GM3 (↑), SOP (↑), TYR (↑), O-MHE (↑) | 0.677 |
| AO090701000885 | α-L-arabinofuranosidase (axhA) | GH-62 | O-CHE (↑), O-X3G4R (↑), O-BGHEXA (↑), SOP (↑) | 0.719 |
| AO090701000838 | α-L-arabinofuranosidase | GH-43 | O-CHE (↑),O-X3G4R (↑), O-BGHEXA (↑), O-GMH (↑), O-AHP (↑), O-GM3 (↑), SOP (↑), TYR (↑), O-MHE (↑) | 0.895 |
| AO090012000445 | β-galactosidase (LacA) | GH-35 | O-CHE (↑), O-X3G4R (↑), O-GMH (↑), SOP (↑), TYR (↑), O-MHE (↑) | 0.713 |
| AO090023000053 | β-galactosidase or α-L-arabinofuranosidase B | GH-2 | O-CHE (↑), O-X3G4R (↑), SOP (↑), O-MHE (↑) | 0.456 |
| Galacto(gluco)mannan | ||||
| AO090003001410 | β-mannosidase | GH-2 | O-CHE (↑),O-X3G4R (↑), O-BGHEXA (↑), O-AHP (↑), O-GMH (↑), O-AHP (↑), SOP (↑), O-MHE (↑) | 0.675 |
| AO090001000556 | β-mannosidase | GH-2 | O-CHE (↑),O-X3G4R (↑), O-BGHEXA (↑), O-AHP (↑), SOP (↑), TYR (↑) | 0.492 |
| AO090003001305 | α-galactosidase | GH-27 | O-CHE (↑), O-GM3 (↑) | 0.597 |
| Pectic polysaccharides | ||||
| AO090026000252 | Rhamnogalacturonase | GH-28 | O-CHE (↑),O-X3G4R (↑), O-BGHEXA (↑), SOP (↑), O-MHE (↑) | 0.82 |
| AO090003001268 | Rhamnogalacturonan acetylesterase | CE-12 | O-CHE (↑) | 0.804 |
| AO090005001400 | Exo-polygalacturonase | GH-28 | O-CHE (↑) | 0.858 |
| AO090023000161 | polygalacturonase | GH-28 | O-CHE (↓),O-X3G4R (↓), SOP (↓) | 0.531 |
| AO090138000086 | endo-polygalacturonase | GH-28 | O-CHE (↑), O-X3G4R (↑), O-BGHEXA (↑), SOP (↑), O-MHE (↑) | 0.763 |
| AO090003001017 | exo-α-L-1,5-arabinanase | GH-93 | O-CHE (↑),O-X3G4R (↑), O-BGHEXA (↑), O-AHP (↑), O-GM3 (↑), SOP (↑), TYR (↑), O-MHE (↑) | 0.825 |
| AO090026000804 | endo-arabinase | GH-43 | O-CHE (↑), O-X3G4R (↑), SOP (↑), TYR (↑), O-MHE (↑) | 0.81 |
| Other hydrolases | ||||
| AO090103000218 | Chitinase | GH-18 | O-CHE (↓) | 0.797 |
| AO090005000029 | cutinase (PDB: 3GBS) | CE-5 | O-CHE (↑),O-X3G4R (↑), O-AHP (↑), SOP (↑), O-MHE (↑) | 0.574 |
| AO090011000665 | Cutinase | CE-5 | O-CHE (↑),O-X3G4R (↑), O-BGHEXA (↑), SOP (↑), TYR (↑), O-MHE (↑) | 0.769 |
| AO090023000058 | Cutinase | CE-5 | TYR (↑) | 0.614 |
Regulation is indicated by arrows
aD-score 0.725 when MHTLNMQALVALSPLLFSAATA is considered as signal peptide omitting 87 residues from the N-terminal
bReclassified recently in CAZy database as AA-9 [25]