Table 3.
Expression patterns of differentially expressed miRNAs and targeted mRNA genes analyzed in meristem tip and base tissue of in vitro-grown pear shoots by qRT-PCR and deep sequencing
| ID miRNA | Log2(T37/T24) | q RT-PCR | ID target gene | q RT-PCR | Functional annotation | ||
|---|---|---|---|---|---|---|---|
| Tip | Tip | Base | Tip | Base | |||
| miR397a | Up (1.03) | Up (2.0) | Up (15.9) | Pbr0359622 | Down (0.15) | Up (1.22) | Laccase-4-like |
| miR477b | Down (−1.4) | Down (0.3) | Up (2.39) | Pbr027204 | Up (4.43) | - | DELLA protein RGL1-like |
| miR3627-5p | Up (2.46) | Up (8.8) | - | -- | -- | -- | -- |
| miR5519 | Up (1.06) | Down (0.67) | Down (0.32) | Pbr002489 | Up (1.32) | - | Proteasome subunit alpha type-4 |
| novel262 | Down (−16.35) | Down (0.22) | Up (2.1) | Pbr030437 | Up (1.23) | Up (1.47) | ABC transporter A family member |
| novel177 | Down (−9.02) | Down (0.55) | Up (9.1) | Pbr019211 | Up (3.66) | Up (2.64) | NAC domain-containing protein 72-like |
| novel345 | Down (−12.05) | Down (0.25) | Down (0.5) | Pbr042600 | Up (1.8) | Up (1.04) | 2-oxoglutarate dehydrogenase, mitochondrial-like |
| novel482 | Up (9.66) | Down (0.33) | Up (11.3) | Pbr025376 | Up (1.42) | Down (0.37) | disease resistance protein RGA2-like |
| novel566 | Up (7.2) | Down (0.46) | Down (0.99) | Pbr023226 | Down (0.59) | Up (1.32) | E3 ubiquitin-protein ligase UPL1-like |
| novel188 | Down (−7.54) | Down (0.63) | Up (10.7) | Pbr017710 | Up (2.4) | Up (2.6) | Squamosa promoter-binding-like Protein13A |
| novel197 | Down (−1.21) | Down (0.22) | - | Pbr039336 | - | - | probable methyltransferase PMT24 |
| novel241 | Down (−9.33) | Down (0.74) | - | Pbr004679 | - | - | probable mannitol dehydrogenase |
“-” indicate no detected data by real-time PCR; “--” indicates that miR3627-5p had no predicted target gene