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. 2015 Nov 14;15:251. doi: 10.1186/s12862-015-0532-9

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© Sember et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Karyotypes of male and female of S. fasciolata after different cytogenetic protocols. a female (2n = 50), b-d male (2n = 51). a, c conventional Giemsa staining, b dual-colour FISH with 45S rDNA (red, arrows) and 5S rDNA (green, arrowheads) probes, d C-banding. Putative sex chromosomes of female are boxed b, d next to those of the male karyotype. Note the presence of 5S rDNA site (b) and prominent pericentromeric heterochromatic region (d) on a putative X chromosome. Notice also a centromeric C-band on Y₁ (d). Bar = 10 μm