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. 2015 Dec;21(12):2132–2142. doi: 10.1261/rna.053637.115

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© 2015 Zagalak et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 1. — Synthesis and annealing properties of RNAs containing randomized nucleotide positions. (A) Sequences of the siRNDs 1–6. Watson–Crick base pairs are indicated with a dash. Randomized vicinal positions are indicated with a dot. (B) Deep-sequencing analysis of siRND3 antisense strand shows equal base distribution at nearly all randomized positions. Consensus sequence was created using WebLogo3. (C) Native polyacrylamide gel electrophoresis (PAGE) of siRNDs. Lanes 1,2, and 10 are loaded with single-stranded RNAs from siRND1 and siRND4 and serve as references; lane 9 contains siCon; lanes 3–8 contain siRNDs 1–6. (D) Melting curve analysis of siRNDs. The graph shows the first derivative of melting curves. T_Ms listed in the table represent the mean of triplicate measurements ± SD.