Table 2.
Protein, gene model or transcript IDs for the genes involved in meiosis analyzed in this study
| Protein name | Accession numbers of proteins used as query | Thalassiosira pseudonana protein ID | Phaeodactylum tricornutum protein ID | Fragilariopsis cylindrus protein ID | Pseudo-nitzschia multiseries protein ID | Pseudo-nitzschia multistriata gene model ID | Seminavis robusta Transcript ID |
|---|---|---|---|---|---|---|---|
| DNA replication and chromosome maintenance | |||||||
| Mcm2 | NP_001185154.1 | 29936 | 18622 | 204899 | 209470 | 0061270.1 | Semro_comp78811_c0_seq1 |
| Mcm3 | Q9FL33.1 | 34975 | 51597 | 264318 | 318351 | 0004850.1 | Semro_comp50104_c0_seq1 |
| Mcm4 | NP_179236.3 | 269123 | 51412 | 146869 | 203268 | 0078600.1b | Semro_comp82592_c0_seq1 |
| Mcm5 | NP_001189521.1 | 31609 | 11490 | 224321 | 255529 | 0118810.1 | Semro_comp83065_c1_seq1 |
| Mcm6 | AED95141.1 | 26545 | 468 | 193082 | 321321 | 0022580.1 | Semro_comp61600_c0_seq1 |
| Mcm7 | P43299.2 | 262526 | 13243 | 184349 | 243980 | 0109820.1 | Semro_comp70058_c0_seq2 |
| Mcm8 | NP_187577.1 | 261512 | 52561 | 189062 | 213178 | 0068370.1 | Semro_comp79168_c0_seq3 |
| Mcm9 | NP_179021.3 | 37362 | 981 | 156569 | 183315 | 0056900.1 | Semro_comp59174_c0_seq1 |
| Smc1 | AEE79265.1 | 35499 | 25506 | 212269 | 162817 | 0116990.1 | Semro_comp83927_c0_seq1 |
| Smc2 | NP_201047.1 | 1393 | 30352 | 210755 | 191984 | 0096310.1 | Semro_comp61213_c0_seq1 |
| Smc3 | NP_180285.4 | 259020 | 52607 | 208027 | 251818 | 0079810.1 | Semro_comp78328_c0_seq1 |
| Smc4 | AED95695.1 | 42365 | 44165 | 212991 | 144962 | 0030660.1 | Semro_comp76089_c0_seq1 |
| Smc5 | AED92224.1 | 9851 | 54192 | 193562 | 286374 | 0102810.1 | Semro_comp64598_c0_seq1 |
| Smc6 | NP_196383.1 | 1743 | 36853 | 177172 | 165557 | 0090050.1 | Semro_comp65344_c0_seq1 |
| Pds5 | NP_177883.5 | 5929 | 1590 | 136077 | 203285 | 0089060.1 | Semro_comp82484_c0_seq6 |
| Scc3 | AEC10920.1 | 8747 | 51870 | 234878 | 38865 | 0079350.1 | Semro_comp20575_c0_seq1 |
| Rec8/Syn1a | NP_196168.1 | NF | NF | NF | NF | NF | NF |
| Rad21 | NP_851110.1 | 8557 | 44595 | 245879 | 324402 | 0072170.1 | Semro_comp80503_c0_seq6 |
| Double-strand break formation | |||||||
| Spo11-1a | AEE75304.1 | NF | NF | NF | NF | NF | NF |
| Spo11-2a | AEE34178.1 | 263510 | 36531 | 242364 | 156625 | 0108120.1b | Semro_comp74200_c0_seq1 |
| Spo11-3/Top VIA | NP_195902.1 | 42646 | 24838 | 239125 | 251788 | 0081370.1 | Semro_comp59497_c0_seq2 |
| DNA damage sensing and response | |||||||
| Rad50 | AEC08614.1 | 9195 | 51876 | 243939 | 320939 | 0001820.1 | Semro_comp61512_c0_seq1 |
| Mre11 | NP_200237.1 | 34332 | 54699 | 275781 | 233741 | 0086370.1 | Semro_comp82091_c0_seq3 |
| Xrs2/Nbs1 | ABA54896.1 | NF | NF | NF | NF | NF | NF |
| Crossover regulation | |||||||
| Mer3a | AAX14498.1 | 11979 | 39994 | 239915 | 285411 | 0087420.1 | Semro_comp60890_c0_seq1 |
| Hop1/Asy1a | AEE34638.1 | NF | NF | NF | NF | NF | NF |
| Red1/Asy3a | AEC10782.1 | NF | NF | NF | NF | NF | NF |
| Zip1/Zyp1a | AEE30217.1 | NF | NF | NF | NF | NF | NF |
| Zip2a | NP_011265.1 | NF | NF | NF | NF | NF | NF |
| Zip3/Hei10a | NP_175754.2 | NF | NF | NF | NF | NF | NF |
| Zip4a | ABO71664.1 | NF | NF | NF | NF | NF | NF |
| Dmc1a | AAC49617.1 | NF | NF | NF | NF | NF | NF |
| Hop2a | CAF28783.1 | NF | NF | NF | NF | NF | NF |
| Mnd1a | NP_194646.2 | 25513 | 54296 | 273989 | 295346 | 0080640.1 | Semro_comp20014_c0_seq1 |
| Msh4a | AAT70180.1 | 261368 | 51916 | 144820 | 259109 | 0116300.1b | Semro_comp57561_c0_seq2 |
| Msh5a | NP_188683.3 | 16039 | 52173 | 149505 | 183820 | 0023810.1 | Semro_comp80580_c0_seq6 |
| Double-strand break repair (recombinational repair) | |||||||
| Rad51-A | BAE99388.1 | 261303 | 51999 | 165795 (A1) 197408 (A2) | 212272 (A1) 1352 (A2) | 0056780.1 (A1) 0086180.1 (A2) | Semro_comp76648_c0_seq1 |
| Rad51-B | NP_180423.3 | 261577 | 40092 | 241710 | 325273 | 0105810.1 | Semro_comp71219_c0_seq1 |
| Rad51-C | CAC14091.1 | 257784b | 54137 | 201530 | 29459 | 0104040.1 | Semro_comp71710_c0_seq3 |
| Rad51-D | NP_001077479.1 | NF | NF | NF | NF | NF | NF |
| Xrcc2 | NP_201257.2 | NF | NF | NF | NF | NF | NF |
| Xrcc3 | NP_200554.1 | 2081c | 31781 | 242664c | 292867c | comp26486_c0_seq1c | Semro_comp70556_c0_seq2c |
| Rec-A | BAE99388.1 | 267595 | 51425 | 186275 | 166360 | 0063260.1 | Semro_comp77000_c0_seq1 |
| Rad52 | CAA86623.1 | 25447 | 49083 | 238228 | 50181 | 0088620.1 | Semro_comp79910_c0_seq1 |
| Rad1 | Q9LKI5.2 | 22869 | 30908 | 208467 | 230429 | 0074360.1b | Semro_comp82187_c0_seq2 |
| Msh2 | AEE76112.1 | 32661 | 19604 | 159571 | 153636 | 0083140.1 | Semro_comp80478_c0_seq1 |
| Msh6 | NP_001190656.1 | 261781 | 53969 | 212924 | 190397 | 0084190.1 | Semro_comp80580_c0_seq6 |
| Mlh1 | NP_567345.2 | 263509 | 54331 | 136590 | 257081 | 0125040.1 | Semro_comp75421_c0_seq1 |
| Mlh2 | NP 013135.1 | NF | NF | NF | NF | NF | NF |
| Mlh3 | NP_195277.5 | NF | NF | NF | NF | NF | NF |
| Pms1 | AAM00563.1 | 264783 | 14607 | 248102 | 242883 | 0117080.1 | Semro_comp73186_c0_seq1 |
| Mms4/Eme1 | AAF06816.1 | NF | NF | NF | NF | NF | NF |
| Mus81 | NP_194816.2 | NF | 36625 | 241086 | 63674 | 0100930.1b | Semro_comp84506_c0_seq2 |
| Fancm | NP_001185141.1 | 11922 | 47619 | 248113 | 68428 | 0010100.1 | Semro_comp74927_c0_seq1c |
| Accessory proteins required during meiosis | |||||||
| Fen1 | AED93576.1 | 269347 | 48638 | 206746 | 260195 | 0006800.1 | Semro_comp51200_c0_seq1 |
| Exo1 | Q8L6Z7.2 | 4742 | 48206 | 261553 | 110816 | 0067080.1 | Semro_comp61722_c0_seq1 |
| Dna2 | NP_001184943.1 | 10652 | 35426 | 241656 | 326992 | 0027070.1 | Semro_comp83726_c0_seq1 |
| Brca1 | AAO39850.1 | NF | NF | NF | NF | NF | NF |
| Brca2 | AEE81814.1 | 6763 | 36784 | 253990 | 284242 | 0067160.1 | Semro_comp82255_c0_seq4 |
Genes marked with a are genes that do not have known functions outside meiosis. Arabidopsis thaliana meiotic proteins were used as query sequence; whenever the specific query gene was not found/present in A. thaliana, Saccharomyces cerevisiae proteins were used (accession numbers in bold). Protein IDs are given for the diatom genomes available at the JGI portal, gene models IDs are given for the Pseudo-nitzschia multistriata genome and transcripts IDs for Seminavis robusta. Actual gene model IDs for P. multistriata include the prefix PSNMU-V1.4_AUG-EV-PASAV3
bcorrected gene model, NF not found, cgene model might need validation