Table 5.

In silico predictions obtained for variants of unknown significance in the South African cohort

					In silico prediction programs
Ethnicity	Variant	Gene	Amino acid change	Occurrence	Classification	Align GVGDa	SIFT	Mutation Taster	PolyPhen	Refs
Black	c.1843_1845delTCT	BRCA1	p.Ser615del	1	3	-	-	-	-	[58–60]
Black	c.4798_4800delAAT	BRCA2	p.Asn1600del	1	3	-	-	-	-	[61]
Black	c.7712A > G	BRCA2	p.Glu2571Gly	1	3	C0	Deleterious	Disease causing	Probably damaging	[62]
Black	c.9875C > T	BRCA2	p.Pro3292Leu	2	3	C0	Affect protein function	Disease causing	Probably damaging	[63]
Black	c.118A > G	PALB2	p.Arg40Gly	1	3	C0	Affect protein function	Polymorphism	Probably damaging	Novel
Black	c.2845 T > C	PALB2	p.Cys949Arg	1	3	C0	Affect protein function	Disease causing	Probably damaging	Novel

^aSpectrum of prediction classes (C0, C15, C25, C35, C45, C55, C65) with C0 less likely to be deleterious and C65 most likely