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. 2015 Nov 14;15:901. doi: 10.1186/s12885-015-1916-3

Fig. 2.

Fig. 2

GAS6-mediated AXL activation increases protumorigenic properties of LPS cells in vitro. a DDLPS (Lipo-246 and Lipo-863) or PLS (LiSa2 and PLS-1) cells were incubated with low-serum media for 24 h prior to a 15 min stimulation with 400 ng/mL GAS6 and analyzed via western blotting. b Changes in DDLPS and PLS cell proliferation were measured by MTS assays with or without GAS6 stimulation for 48 h. Percent of cell growth is expressed as a percentage of control. c Modified Boyden chambers were used to assess the effects of GAS6 stimulation on cell migration and invasion. Representative images of each condition are shown (200× magnification). Percent of cell migration and invasion is expressed as a percentage of control. The data in the bar graphs are means with SEMs for triplicate experiments (* = p < 0.05; *** = p < 0.001)