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. 2015 Nov 14;15:901. doi: 10.1186/s12885-015-1916-3

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© May et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Transient knockdown of AXL decreases cell proliferation, migration, and invasion. a Representative western blots showing signaling dysregulation in Lipo-246, Lipo-863, LiSa2, and PLS-1 cells that were transfected with either NT or AXL-specific siRNA. b Cell proliferation of AXL-specific siRNA-transfected DDLPS and PLS cell lines was measured by MTS assay after 48 h (96 h post-transfection). Percent of cell growth is expressed as a percentage of control. c Modified Boyden chambers were used to assess DDLPS and PLS cell migration and invasion following transfection with AXL-targeting siRNA. Percent of cell migration and invasion is expressed as a percentage of control. The data in the bar graphs are means with SEMs for triplicate experiments (* = p < 0.05; *** = p < 0.001)