Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Nov 1;29(21):2244–2257. doi: 10.1101/gad.268128.115

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Kim et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 4. — WSB1 up-regulates HIF-1α activity. (A) HEK293T cells were transfected with the indicated constructs, and HIF-1α level was examined. (Exo) Exogenous; (Endo) endogenous. (B,C) Quantitative PCR (qPCR) analyses of selected HIF-1α targets in HEK293T cells stably transfected with WSB1 cDNA (B) or WSB1 shRNA (C). Expression levels are relative to β-actin; data were normalized to control cells. The results represent the means ± SE of three independent experiments performed in triplicate. (*) P < 0.05; (**) P < 0.01; (***) P < 0.001 versus control cells by one-way ANOVA. (D) The corelation between WSB1 and HIF target gene expression in metastatic melanoma (n = 50), prostate cancer (n = 25), and breast cancer (n = 34) patients (Gene Expression Omnibus data set).