Fig. 1.

PFN1 protein or mRNA levels are not affected by the PFN1 T109M mutation. a Measurement of relative PFN1 mRNA abundance by qRT-PCR of LCLs of five healthy controls and PFN1 T109M mutant LCLs of two ALS patients and three pre-clinical mutation carriers. Results were normalized to U6 snRNA using 2^−ΔΔCt-method (bars indicate mean ± SEM). b Western blot comparing amounts of PFN1 protein of LCLs of five healthy controls, two PFN1 T109M mutant ALS patients and three pre-clinical carriers of the PFN1 T109M mutation. Same amounts of protein were pooled in the respective group. Actin is shown as a loading control