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. 2015 Nov 16;16:77. doi: 10.1186/s12868-015-0214-y

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© Freischmidt et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — The T109M mutation of PFN1 does not change the F/G-actin ratio in HEK293 cells. Upper panel F/G-actin ratios of HEK293 cells overexpressing wildtype and mutant V5-PFN1. F- and G-actin fractions of the respective cell lysates were separated by ultracentrifugation and actin levels analyzed by Western blotting and densitometry (n = 5; bars indicate mean ± SEM; * p ≤ 0.05 in a two tailed student’s t test). Lower panel Representative Western blot of F- and G-actin fractions used for densitometric analysis