Table 1.

Oligonucleotides used for generation of PFN1 T109M, T109A and T109D by site directed mutagenesis

Mutation	Sequence
T109M	5′-CTGTCACCAAGACTGACAAGATGCTAGTCCTGCTGATGGGC-3′
	5′-GCCCATCAGCAGGACTAGCATCTTGTCAGTCTTGGTGACAG-3′
T109A	5′-CTGTCACCAAGACTGACAAGGCGCTAGTCCTGCTGATGGGC-3′
	5′-GCCCATCAGCAGGACTAGCGCCTTGTCAGTCTTGGTGACAG-3′
T109D	5′-CTGTCACCAAGACTGACAAGGATCTAGTCCTGCTGATGGGC-3′
	5′-GCCCATCAGCAGGACTAGATCCTTGTCAGTCTTGGTGACAG-3′