Fig. 3.

PTEN suppresses TH phosphorylation and stability via inhibition of the ERK/MAPK pathway in PC12 cells. a Western blot of the named proteins/phosphoproteins after exposure of NGF (to activate PC12 cells from resting state) or not, in the presence (Tet−) or absence (Tet+) of exogenous PTEN expression (left panel). Note that expression of PTEN reduces TH phosphorylation in PC12 cells. Normalized quantitation of relative TH and P-TH expression in the presence (white bar, Tet−) or absence (black bar, Tet+) of exogenous PTEN expression with (NGF+) or without (NGF−) exposure (right bar graphs). b Western blot of the named proteins/phosphoproteins in the presence (+) or absence (−) of MAPK inhibitor PD98059 or of PI3K inhibitor LY294002 with (+) or without (−) NGF stimulation of PC12 cells. Note that MAPK inhibitor PD98059, but not PI3K inhibitor LY294002, is associated with decreased P-TH even after NGF stimulation