Skip to main content
. 2010 Jul 9;31(9):1561–1566. doi: 10.1093/carcin/bgq143

Fig. 2.

Fig. 2.

miR-663 decreases AP-1 activity and targets JunB. (A) AP-1 activity was measured in extracts of THP-1 cells transfected with the AP-1 luciferase reporter plasmid and with either a Control RNA, pre-miR-663 (663) or a miR-663 inhibitor RNA (663-I), with or without LPS challenge (6 h). Values represent the mean ± standard deviation (n = 3). * and **, significantly different from the corresponding mock-challenged sample, *P < 0.001 and **P < 0.0005. o, significantly different from LPS-challenged Control, P < 0.0008. #, significantly different from mock-challenged Control, P < 0.0001. (B) The levels of JunB in THP-1 cells transfected with either a pre-miR Control, pre-miR-663 or a miR-663 inhibitor RNA (663-I), with or without LPS challenge (6 h), were determined by western blotting. (C) Effects of pre-miR-663 on Luc-JunB constructs, both in sense and antisense orientation. Sequences of the two mutated miR-663 target sites in JunB sense constructs (M663) are given in supplementary Table II, available at Carcinogenesis Online. Values represent the mean ± standard deviation (n = 4). * and **, significantly different from Pre-miR-Control, *P < 0.001 and **P < 0.0001.