Fig. 7.

Effects of various cations on NO decay rate and GSNO yield. (A) Apparent pseudo-first-order rate constants in the absence and presence of various cations. (B) Corresponding GSNO yields from 0.75 µM NO and 2 mM GSH. Experimental conditions: 1 µM PROLI/NO, 2 mM GSH, 4 mM CuSO₄, 1000 U/ml SOD, 0.1 mM DTPA, 5 mM indicated cation, and 50 mM TEA (pH 7.4) at 37 °C. N.D., the yield in the presence of Al³⁺ could not be determined, as Al³⁺ also stimulated GSNO formation from GSH and nitrite. Data are shown ±SEM (n=5).