Table 1.

Comparison of GSNO yields determined by various methods.

Method	GSNO (µM)
+Mg2+	−Mg2+
Electrode	0.76±0.02	0.37±0.03
NO analyzer	0.75±0.03	0.35±0.04
HPLC	0.70±0.05	0.29±0.04

Experimental conditions: 1 µM PROLI/NO (~0.75 µM NO), 5 mM GSH, 1000 U/ml SOD, 0.1 mM DTPA, 5 mM MgCl₂ as indicated, and 50 mM TEA (pH 7.4) in 0.5 ml at 37 °C. Data are shown ±SEM (n=3–5).