Table 2.
Yields of GSNO from the reaction of GSH and various NO donors.
| Donora (1 µM) | t1/2 (s) | Pre-Cu2+ NO peak (µM) | GSNO (µM) |
|
|---|---|---|---|---|
| −Mg2+ | +Mg2+ | |||
| SPER/NO | 1806±6 | n.d. | 0.89±0.05 (~56%) | 1.19±0.09 (~74%) |
| DEA/NO | 67±3 | 0.70±0.03 | 0.47±0.05 (~32%) | 0.94±0.14 (~65%) |
| PROLI/NO | 1–2 | 1.21±0.02 | 0.38±0.01 (~25%) | 0.86±0.15 (~57%) |
Experimental conditions: 1 µM SPER/NO, DEA/NO, or PROLI/NO; 1 mM GSH; 1000 U/ml SOD; 0.1 mM DTPA; 0 or 5 mM MgCl2; and 50 mM TEA (pH 7.4) in 0.5 ml at 37 °C.
a
SPER/NO, DEA/NO, and PROLI/NO released 1.60±0.03, 1.46±0.02, and 1.52±0.06 µM NO under the conditions used as determined by the conversion of oxyhemoglobin to methemoglobin. n.d.: not detectable. Data are shown ±SEM (n=5–7).