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. 2015 Nov 17;5:16641. doi: 10.1038/srep16641

Figure 8. Structural validation of the last three steps of the proposed reaction mechanism for the hydrolysis of SAH to HCY and ADO catalysed by SAHH.

Figure 8

Hydrogen atoms that transfer as proton ions are shown in blue, cyan, and green. Hydrogen that transfers as a hydride ion is shown in red. (A) Step 4: the addition of water to the C4′-C5′ double bond of 3′-keto-4′,5′-didehydroadenosine through a Michael-type addition (left). The MmSAHH/NAD+/NRN complex (right) is a structural homolog of this intermediate. (B) Step 5: the reduction of the 3′-keto group of 3′-keto ADO to form ADO (left). The MmSAHH/NADH/3KA complex (right) is a structural homolog of this intermediate. (C) Reaction product: the binding mode of the reaction product just after step 5 (left). The MmSAHH/NAD+/ADO complex (right) represents this state.