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. 2007 Jun 1;129(5):879–890. doi: 10.1016/j.cell.2007.03.043

Figure 4.

Figure 4

DAPK1 Expression in CLL Family 4532

(A) Pedigree of CLL family no. 4532. Open circle and square represent unaffected female and male, respectively, while closed circle and square represent affected female and male.

(B) Sequencing of SNP c.114A > G from genomic DNA and cDNA of two unaffected (IV-3 and IV-4) and two affected (III-3 and III-4) individuals.

(C) RT-PCR on RNA isolated from monochromosomal hybrid clones with either WT or the CLL chromosome 9 from fibroblast cells of individual III-4 is shown. RPL4 expression was used as an internal control. Error bars indicate SD.

(D) DAPK1 protein expression from WT and CLL alleles in monochromosomal hybrids. Jurkat and WaC3CD5 cells were used as positive controls, and NIH3T3 cells were used as the negative control to show specificity to human DAPK1.

(E and F) The RT-PCR product amplifying SNP c.1510A > G in unaffected (IV-3; E) and affected (III-4; F) fibroblast cell lines was cloned, and individual clones were genotyped. Shown is the percentage of A and G clones in IV-3 and III-4, and n is the number of clones studied. The difference in allelic expression of DAPK1 in III-4 and IV-3 was statistically significant (p < 0.01).