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Effect of signaling inhibitors on the CXCL1 mRNA level. HUVECs were pretreated with SP600125 (SP), SB202190 (SB), LY294002 (LY), or genistein (Gen) (10 μmol/L each) for 1 h, followed by stimulation with TNF-α (5 ng/mL) for 1.5 h. Total RNA was extracted and analyzed by RT-PCR. The data are the mean±SEM (n=3). bP<0.05 vs TNF-α and vehicle treatment.
