Table 1.
Entry | [NAD+] [mm] | Conversion [%] | Initial activity[a] | Total turnover number[b] |
---|---|---|---|---|
1 | 0.07 | >97[c] | 1.4±0.1 | 1250[c] |
2 | 2.00 | 9.3[d] | 7.8±0.5 | 53 100[d] |
3 | 5.00 | 75[e] | N.D. | >94 800[e] |
[a] Initial activity [μmol min−1 per mg NAD+ reductase] measured during the initial linear reaction phase; [b] Total turnover number [moles of NADH generated per mole of NAD+ reductase exposed to the carbon particles]; [c] Measured after 30 min; [d] Measured at 35 min; [e] Measured at 20 h; N.D., not determined.