Table 3.

Comparison of the activity of H₂-driven acetophenone reduction using particles modified with hydrogenase, NAD⁺ reductase and ADH in the presence of different cofactor concentrations

Entry	[NAD+] [mm]	Turnover frequency[a]	Turnover number[b]	Total turnover number[c]	Conversion [%][d]
1	1	1.41	9.85	135 000	>98
2	0.1	0.88	84.4	116 000	84
3	0.01	0.20	655	90 000	66

[a] Moles of phenylethanol per second per mole NAD⁺ reductase recorded over the first 6 h; [b] Moles of phenylethanol per mole cofactor after 21.5 h; [c] Moles of phenylethanol per mole NAD⁺ reductase after 21.5 h; [d] Conversion [%] after 21.5 h. Conditions: samples were shaken during reaction, with H₂ (1 bar) flowing through the headspace; 10 mm acetophenone, Tris-HCl buffer, pH 8, 25 °C.