Table 3.
Entry | [NAD+] [mm] | Turnover frequency[a] | Turnover number[b] | Total turnover number[c] | Conversion [%][d] |
---|---|---|---|---|---|
1 | 1 | 1.41 | 9.85 | 135 000 | >98 |
2 | 0.1 | 0.88 | 84.4 | 116 000 | 84 |
3 | 0.01 | 0.20 | 655 | 90 000 | 66 |
[a] Moles of phenylethanol per second per mole NAD+ reductase recorded over the first 6 h; [b] Moles of phenylethanol per mole cofactor after 21.5 h; [c] Moles of phenylethanol per mole NAD+ reductase after 21.5 h; [d] Conversion [%] after 21.5 h. Conditions: samples were shaken during reaction, with H2 (1 bar) flowing through the headspace; 10 mm acetophenone, Tris-HCl buffer, pH 8, 25 °C.