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. 2015 Sep 9;54(45):13198–13203. doi: 10.1002/anie.201506112

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© 2015 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

© 2015 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Scheme 1 — Amino acids of CRM₁₉₇ were derivatized with a different average number of linkers per protein. Appropriate bifunctional azido linkers were used according to the amino acids targeted on the protein. See the Supporting Information for detailed reaction conditions.