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. 2015 Nov 17;5:16877. doi: 10.1038/srep16877

Figure 1. rP21 treatment increased IL-4 expression in polyester sponge-induced inflammation.

Figure 1

rP21 and bacterial extract (B.E.) down-regulated interleukin-1β (IL-1β) (A) and tumor necrosis factor-α (TNF-α) (B). B.E. inhibited IL-4 expression and rP21 treatment augmented this cytokine levels (C). 40 μg/mL of rP21 and B.E. were used. The experiment was performed twice using 10 animals/group. Samples were analyzed individually in triplicate. Data are expressed as mean ± standard deviation. Significant differences were determined by Dunn’s test (GraphPad Prism software, version 6.01). Differences were considered significant when p < 0.05. A 12% Coomassie Blue stained Sodium dodecyl sulfate-Polyacrylamide gel electrophoresis (SDS-PAGE) gel is shown to demonstrate de purity of purified rP21 and the bacterial protein pattern in B.E. (D). MW: molecular weight expressed in kilo Daltons (kDa). rP21 = 18 kDa.