Figure 3. mRNA expression of DMT1, FPN1, DcytB and HP in intestinal mucosa.
The levels of mRNA of DMT1 (A), FPN1(C), DcytB (D) and HP (E) were determined in intestinal mucosa by qRT-PCR (controls: n = 16, terminal ileum CD: n = 18, colonic CD: n = 20, colonic UC: n = 17). (B) Paired intestinal mucosal biopsies were taken from normal or inflamed mucosa from the same patients with A-CD (n = 12), and mRNA levels of DMT1 were analyzed by qRT-PCR. (F) Biopsies were taken from 15 patients with CD, 11 patients with UC, 10 healthy controls. The levels of mRNA for DMT1 were detected by qRT-PCR. Gene expression was normalized to GAPDH mRNA levels in each sample. (A,D,E) Kruskal-Wallis test, (B) Student’s t test, (C,F) one way ANOVA. *P < 0.05, **P < 0.01 versus controls (Con). Data are expressed as mean ± SEM. The data are a representative of 3 independent experiments.