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. 2015 Nov 17;5:16344. doi: 10.1038/srep16344

Figure 5. TNF down-regulates the DMT-1 expression in vitro, but rescued by JNK inhibitor.

Figure 5

(A) HCT116 cell line was cultured and stimulated with TNF (20 ng/mL), LPS (100 ng/mL) and IFN-γ (20 ng/mL) in vitro. Cells were collected after 24 and 48 h of culture, and mRNA levels of DMT1 expression were then determined by qRT-PCR. One way ANOVA was performed for statistical analysis. **P < 0.01 versus the data of medium alone (NC). (B) HCT116 cells were incubated with TNF (20 ng/mL) in the presence of JNK inhibitor (JNK-IN-7, 1 μM), NF-κB inhibitor (BAY 11-7082, 1 μM), and caspase-3/8 inhibitor (Z-DEVD-FMK, 50 μM), respectively, as indicated. Cells were then harvested after 48 h of culture, and DMT1 mRNA expression was then determined by qRT-PCR. One way ANOVA was performed for statistical analysis. *P < 0.05 versus the data of control group. Data are expressed as mean ± SEM. Gene expression was normalized to GAPDH mRNA levels in each sample. The data are a representative of 3 independent experiments.