Table 2. PERMANOVA analysis on Bray-Curtis distance for faecal microbiota structure on the genera level from three different collections and six different storage conditions (permutations = 9999).
| Source | df | SS | MS | Pseudo-F | P(perm) |
|---|---|---|---|---|---|
| Storage | 5 | 3184.9 | 636.99 | 18.236 | 0.0001 |
| Collections | 2 | 2420.9 | 1210.4 | 34.653 | 0.0001 |
| Storage x Collections | 10 | 948.13 | 94.813 | 2.7144 | 0.0001 |
| Residual | 36 | 1257.5 | 34.93 | ||
| Total | 53 | 7811.4 | |||
| Comparison | t | P(perm) | |||
| −80 °C Freezer vs 4 °C Fridge | 1.7381 | 0.0491 | |||
| −80 °C Freezer vs OMNIgene.GUT | 2.9592 | 0.001 | |||
| −80 °C Freezer vs RNAlater | 4.6425 | 0.001 | |||
| −80 °C Freezer vs TE Buffer | 5.6022 | 0.001 | |||
| −80 °C Freezer Room Temperature | 3.3297 | 0.001 | |||
df = degree of freedom; SS = sum of squares; MS = mean sum of squares; Pseudo-F = F value by permutation; t = t-statistic.