. 2015 Nov 12;21:3474–3482. doi: 10.12659/MSM.894568

Table 1.

Primers used for amplification of the VEGF genetic polymorphisms.

SNP	Alias name	Annealing Temperature (°C)	Primers for PCR amplification	Restriction enzyme
rs699947	−2578C>A	53	F: 5′-CATACCGATGGAACTGG-3′	Bg1II
rs699947	−2578C>A	53	R: 5′-GTTTCTGACCTGGCTATTT-3′	Bg1II
rs2010963	−634C>G	67	F: 5′-CGACGGCTTGGGGAGATTGC-3′	BsmFI
rs2010963	−634C>G	67	R: 5′-GGGCGGTGTCTGTCTGTCTG-3′	BsmFI
rs3025039	936C>T	53	F: 5′-AGGGTTTCGGGAACCACATC-3′	NlaIII
rs3025039	936C>T	53	F: 5′-AGGGTTTCGGGAACCACATC-3′	NlaIII

SNP – single nucleotide polymorphism; PCR – polymerase chain reaction; F – forward; R – reward.