Figure 2.

The effect of miR-137 down-regulation on H₂O₂ -induced apoptosis in cardiomyocytes. Cardiomyocytes were transfected with lentiviruses of mmu-miR-137 inhibitor (miR-137-In) or its negative control miRNA (miR-C). (A) Twenty-four hours after lentiviral transfection, qRT-PCR was performed to measure the efficacy of lentiviral infection (* P<0.05). (B) Cardiomyocytes were then treated with 100 μM H₂O₂ for another 24 h, followed by a TUNEL assay to evaluate the effect of miR-137 down-regulation on cardiomyocyte apoptosis. (C) The percentages of non-apoptotic cardiomyocytes (DAPI-positive/TUNEL-negative) were compared between the cardiomyocytes transfected with miR-C and the ones transfected with miR-137-In (* P<0.05).