Figure 4.

CDC42 down-regulation reversed the effect of miR-137 on H₂O₂-induced cardiomyocyte apoptosis. (A) Cardiomyocytes were transfected with CDC42 specific siRNA (CDC42-siRNA) or its negative control siRNA (C-siRNA) for 24 h. QRT-PCR was used to evaluate the transfection efficiency (* P<0.05). (B) Cardiomyocytes were transfected with miR-137-In for 24 h, followed by another 24 h transfection of either CDC42-siRNA or C-siRNA. Then, cardiomyocytes were treated with 100 μM H₂O₂ for 24 h, followed by a TUNEL assay to evaluate the effect of CDC42 down-regulation on cardiomyocyte apoptosis. (C) Western blotting analysis was ALSO used to compare the protein expression levels of CDC42, caspase-3 and caspase-9.