Figure 7.

Ventricular mRNA expression of the PKA targets RYR2, TnI, and PLB. (A) RYR2 expression was similar in all genotypes. (B) TnI expression was significantly reduced in β₁-tg/Gα_i2^−/− ventricles compared with wild-type (C57BL/6) tissue. (C) In both β₁-adrenoceptor overexpressing mouse lines (β₁-tg and β₁-tg/Gα_i2^−/−), ventricular PLB expression was significantly reduced compared with wild-type (C57BL/6) tissue. The number of animals used for analysis was 3–4 for each genotype. Age range of examined mice was 288 ± 9 days. Asterisks indicate significant differences compared with mRNA expression levels of age-matched wild-type mice in an REST^® analysis (*P < 0.05).