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. Author manuscript; available in PMC: 2015 Nov 17.
Published in final edited form as: Eur Respir J. 2009 Aug;34(2):489–506. doi: 10.1183/09031936.00042409

FIGURE 2.

FIGURE 2

Sequencing of DNA from cells recovered in a pleural effusion specimen obtained at the time of acquired erlotinib resistance. After PCR amplification of exon 20, 454 sequencing revealed the presence of the T790M resistance mutation of epidermal growth factor receptor (EGFR) [49] at ~2%. Robust calling of T790M carrying EGFR alleles was ensured by >60,000 × over-sampling. Wt: weight; Mut.: mutation. ——: coverage; green: A; blue: C; black: G; red: T. Reprinted from [68], with permission from the publisher.