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. Author manuscript; available in PMC: 2015 Nov 17.
Published in final edited form as: Clin Cancer Res. 2012 Jan 6;18(3):654–665. doi: 10.1158/1078-0432.CCR-11-1406

Figure 1.

Figure 1

Notch pathway components are expressed in uveal melanoma cell lines and primary tumors. A, mRNA levels of Hes1 were analyzed by quantitative PCR in 30 snap-frozen primary tumors, 5 uveal melanoma cell lines, and normal cutaneous melanocytes (NMEL). B, mRNA transcripts of additional Notch receptors, ligands, and target genes were detected in the 30 primary uveal melanomas (T) and in normal epidermal melanocytes (NMEL; top); Pearson correlation coefficient R was determined between Hes1 and Notch1, Notch2 mRNA levels in the primary uveal melanomas (bottom). C, nuclear Hes1 immunoreactivity was examined in 73 tumors collected on a tissue microarray with strong Hes1 immunostaining shown in the left and a no primary antibody negative control on the right. D, examination of mRNA expression of the Hes1, Hes5, Hey1, Hey2 genes in 5 uveal melanoma cell lines and normal cutaneous melanocytes revealed that three uveal melanoma lines had elevated levels of multiple Notch targets.