Fig. 1.
Expression of CCL17 and CCL22 in NNKTL cell lines. a Chemokine protein array analysis of CCL17 and CCL22 expression in NNKTL cell lines (SNK-6, SNT-8, and SNK-1) and KHYG-1 cells. The table shows the NNKTL cell/KHYG-1 expression ratios, which were calculated as spot intensity of NNKTL cells divided by spot intensity of KHYG-1 cells. The production of CCL17 was 183-fold higher in SNK-6, 101-fold higher in SNT-8, and 183-fold higher in SNK-1 compared with KHYG-1. The production of CCL22 was 13.8-fold higher in SNK-6, 2.11-fold higher in SNT-8, and 12.8-fold higher in SNK-1 compared with KHYG-1. b Supernatants from the indicated cell cultures (2.5 × 105/mL) in 96-well round-bottomed plates were collected after 24, 48, and 72 h, and CCL17 and CCL22 production was assessed using ELISA. Columns, means of triplicate determinations; bars, SD