Skip to main content
. Author manuscript; available in PMC: 2016 Jul 20.
Published in final edited form as: J Genet Genomics. 2015 Jun 24;42(7):355–371. doi: 10.1016/j.jgg.2015.06.003

Fig. 6.

Fig. 6

SPINT2 is silenced by DNA methylation in GBM.

A: Bisulfite pyrosequencing confirmed the RRBS methylation results at four CpG sites within the promoter of SPINT2. Each panel shows the comparison between bisulfite sequencing and RRBS data for the corresponding CpG site. B: qRT-PCR analysis of SPINT2 mRNA level revealed that SPINT2 was expressed at various parts of normal brain tissue and the NSC line, but significantly down-regulated in GSC lines 1133S and 1142S. C: SPINT2 was significantly down-regulated in GBMs compared to normal brain tissue samples in TCGA data set. The graph was generated using Oncomine (https://www.oncomine.org/). D: The SPINT2 mRNA expression level was inversely correlated with its promoter methylation status in primary GBM samples based on analyses using TCGA RNA-seq and Illumina 450K methylation array data sets. The correlation plot was generated using cBioPortal (http://www.cbioportal.org/).