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. 2015 Apr 10;22(11):1803–1811. doi: 10.1038/cdd.2015.31

Figure 4.

Figure 4

Normal cell cycle distribution and tumor cell apoptosis in Eμ-Myc tumor cells lacking Raidd. (a) Cell cycle phases G1 (black), S (dotted) and G2/M (white) of lymphoma cells were assessed ex vivo by intracellular DNA content analysis in tumors derived from Eμ-Myc (n=21), Eμ-Myc/Raidd−/− (n=22), Eμ-Myc/Casp-2−/− (n=10) mice. (b) To define the percentage of cells in M-phase, ex vivo tumor cells were stained with an antibody specific for the phosphorylated variant of Histone H3 (pH3) and propidium iodide. Bars represent mean values of pH3+ cells±S.E.M. Eμ-Myc (n=17), Eμ-Myc/Raidd−/− (n=11) Eμ-Myc/Casp-2−/− (n=5). (c) Apoptosis in situ was assessed by Sub-G1 analysis of freshly isolated lymph node tumor masses followed flow cytometric analysis (wt n=24, Raidd−/− n=22, Casp-2−/− n=10) mean values±S.E.M. (d) Freshly isolated lymphoma cells were cultivated on feeder cells for 24 h and were either left untreated (upper left graph) or exposed to increasing doses of the indicated chemo-therapeutics. Cell death was assessed by Annexin-V/7-AAD staining combined with anti-CD19 to identify B cells. Symbols represent mean values, n>3–7 for each genotype±S.E.M.