Figure 6.

Idh1 KO hepatocytes accumulate ROS even at steady state. (a) Flow cytometric determination of ROS levels in steady-state WT and Idh1 KO hepatocytes that were stained with DCFDA. Data are representative of three cultures per group and three experiments. (b) Quantitation of the intracellular NADP⁺/NADPH ratio in steady-state hepatocytes (left) and sublethal LPS-injected hepatocytes at 6 h (right) of each WT and Idh1 KO mice determined as described in Materials and Methods. *P<0.05 and ***P<0.001. (c) Quantitative PCR analysis of mRNA levels of the indicated ROS-related genes in steady-state WT and Idh1 KO hepatocytes. Data are expressed as the fold change relative to values in WT hepatocytes and are the mean±S.D. (n=3). *P<0.05; **P<0.01; ***P<0.001