Figure 1. Integration of SIMcheck functionalities to the SIM imaging workflow.

Solid lines represent the user’s workflow, and point from the various workflow steps to the applicable functions of SIMcheck. Dashed lines represent where SIMcheck’s output can inform user decisions, either in sample preparation, acquisition settings, reconstruction parameter settings, or system calibration. Colour codes are as follows: blue – raw data checks; green – reconstructed data checks; purple – utilities; red – applicable to expert users only.