Skip to main content
. Author manuscript; available in PMC: 2015 Nov 17.
Published in final edited form as: Sci Signal. 2014 Dec 9;7(355):ra116. doi: 10.1126/scisignal.2005770

FIGURE 6. NRG1 induced cell migration is mediated by YAP.

FIGURE 6

(A) Transwell migration assay of T47D cells expressing pINDUCER10 encoding YAP shRNA or scrambled control. Cells were incubated with DOX (1 μg/ml) for 5 days followed by 3 hours NRG1 (50 ng/ml) pre-treatment then allowed to migrate for 48 hours (from 0.1% to 10% FBS) in the presence of NRG1 and DOX. Percent migration was normalized to scrambled (scr) control without NRG1. Data are means (SD) from 3 experiments.

(B) Migration data from (A) shown as fold-change migration +NRG1/−NRG1. Data are means (SD) from 3 experiments.

(C) Immunoblot of YAP protein knockdown in T47D cells expressing pINDUCER10 encoding YAP shRNA or scrambled control. Cells were treated as in (A). Blots are representative of 3 experiments.

(D) Trypan blue cell viability assay in T47D cells expressing pINDUCER10 encoding YAP shRNA or scrambled control. Cells were treated as in (A). n.s., not significant; *p<0.05; **p<0.01, ***p<0.001.