Table 1.
Real time qRT-PCR analysis of mRNA levels in NE-Ca
| Gene Symbol | Gene Name | Major function category | Relative to TRAMP Vehicle as 100%
|
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|---|---|---|---|---|---|---|---|---|---|
| TRAMP Vehicle | SD | TRAMP AGN | SD | TRAMP D/DA | SD | ANOVA p-value | |||
| Pcna | proliferating cell nuclear antigen (DNA repair ) | Proliferation | 100 | 15 | 39 | 9 | 51 | 19 | 0.001 |
| Fen1 | flap endonuclease 1 (PCNA complex, DNA repair) | Proliferation | 100 | 20 | 47 | 12 | 53 | 18 | 0.001 |
| Stat3 | signal transducer and activator of transcription 3 | Proliferation | 100 | 40 | 27 | 3 | 48 | 27 | 0.008 |
| Syt4 | synaptotagmin IV | Neuron/NE | 100 | 70 | 6 | 2 | 16 | 6 | 0.007 |
| Sox5 | SRY (sex determining region Y)-box 5 | Neuron/NE | 100 | 17 | 44 | 19 | 44 | 11 | 0.001 |
| Fgfr1 | fibroblast growth factor receptor 1 | Angiogenesis | 100 | 12 | 77 | 11 | 88 | 27 | 0.246 |
| Fgf10 | fibroblast growth factor 10 | Angiogenesis | 100 | 62 | 45 | 23 | 37 | 13 | 0.066 |
| Fgfbp3 | fibroblast growth factor binding protein 3 | Angiogenesis | 100 | 68 | 21 | 4 | 55 | 43 | 0.076 |
| Mmp2 | matrix metallopeptidase 2 | Angiogenesis | 100 | 43 | 41 | 19 | 34 | 8 | 0.005 |
| Nos2 | nitric oxide synthase 2, inducible | Angiogenesis | 100 | 29 | 52 | 12 | 171 | 114 | 0.031 |
| Vegf | vascular endothelial growth factor | Angiogenesis | 100 | 32 | 70 | 24 | 115 | 30 | 0.046 |
| Flt1 | FMS-like tyrosine kinase 1 (VEGF receptor) | Angiogenesis | 100 | 26 | 75 | 20 | 108 | 37 | 0.258 |
| Snai2 | snail family zinc finger 2 | EMT-metastasis | 100 | 63 | 23 | 6 | 45 | 51 | 0.089 |
| Twist1 | twist basic helix-loop-helix transcription factor 1 | EMT-metastasis | 100 | 33 | 22 | 5 | 98 | 53 | 0.008 |
| Notch1 | notch 1 | EMT-metastasis | 100 | 50 | 46 | 14 | 87 | 55 | 0.227 |
| Tgfbr2 | transforming growth factor, beta receptor II | EMT-metastasis | 100 | 39 | 48 | 13 | 71 | 47 | 0.157 |
| Mmp12 | matrix metallopeptidase 12 (macrophage) | Invasion-metastasis | 100 | 39 | 38 | 15 | 107 | 103 | 0.072 |
| E- cadherin | cadherin 1 (epithelial specific) | Epithelium | 100 | 116 | 233 | 122 | 116 | 129 | 0.039 |
| Vpp1 | ventral prostate predominant 1 | Epithelium-prostate specific | 100 | 52 | 3088 | 2823 | 1916 | 2124 | 0.001 |
| Cpa3 | carboxypeptidase A3, mast cell | Inflammation | 100 | 56 | 29 | 19 | 31 | 31 | 0.027 |
| Cma1 | chymase 1, mast cell | Inflammation | 100 | 54 | 24 | 9 | 60 | 53 | 0.073 |
| Mcpt6 | mast cell protease 6 | Inflammation | 100 | 52 | 18 | 5 | 60 | 49 | 0.068 |
| Il6st | interleukin 6 signal transducer | Inflammation | 100 | 16 | 61 | 7 | 66 | 24 | 0.009 |
| Rela | v-rel reticuloendotheliosis viral oncogene homolog A | Inflammation | 100 | 18 | 59 | 12 | 102 | 25 | 0.010 |
| uPa | plasminogen activator, urokinase | Invasion | 100 | 33 | 80 | 31 | 73 | 30 | 0.467 |
TRAMP Vehicle, n=4, TRAMP AGN, n=4 and TRAMP D/DA, n=4, respectively. Gene names in bold denote those commonly affected by AGN and D/DA; Bold numbers in AGN column denote those affected only by AGN.