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. 2015 Jul 8;5:11874. doi: 10.1038/srep11874

Figure 5. IL-17A and IL-17E activate c-Raf and p70S6 kinase.

Figure 5

Activation of c-Raf (pSer338) and p70S6 kinase (pThr 389) was analyzed successively by Western Blot (WB) on T47D, MDA-MB468 and IJG-1731 cell lines. Cells were treated with either IL-17A or IL-17E at 20 ng/ml for 20 min before cell lysis. 70 μg of protein were loaded for each condition and loading control was done with an anti β-actin mAb. Data are representative of 3 independent experiments and all the lines were run on the same gel and under the same experimental procedure.