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. 2015 Jul 8;5:11874. doi: 10.1038/srep11874

Figure 6. IL-17A or IL-17E enhance the generation of low molecular weight forms of cyclin E (LMW-E).

Figure 6

LMW-E generation was analyzed by WB on MCF-7, T47D, IJG-1731 and MCF10A after 72 h of treatment with Il-17A or IL-17E at 20 ng/ml. 70 μg of protein were loaded for each condition and loading control was done with an anti β-actin mAb after stripping. Almost all of the five published short forms ranging from 34 to 49 kDa, were induced. No induction was found for the nonmalignant MCF10A cell line suggesting a specific feature of tumor cells. Data are representative of 3 independent experiments and all the lines were run on the same gel and under the same experimental procedure.