Figure 2.

A–B) Ionic currents from Shaker mutants obtained with the same protocol as in 1C. C) Comparison of peak current amplitudes at +60 mV. Error bars indicate SEM with n =10–20 oocytes. D) Conductance-voltage (GV) relationships were fitted to a Boltzmann equation (V_½,M1stop = −19.1 mV, dV_M1stop = 9.9 mV, V_{½,M1stop-M312L} = −23.0 mV, dV_M1stop-M312L = 11.3 mV, V_½,A3stop = −18.1 mV, dV_A3stop = 9.1 mV, V_½,G6stop = −15.5 mV, dV_G6stop = 9.7 mV, V_½,Y81stop = −14.6 mV, dV_Y8stop = 10.8 mV, V_½,G9stop = −13.0 mV, dV_G9stop = 10.0 mV, V_½,K19stop = −16.3 mV, dV_K19stop = 9.3 mV, V_½,E35stop = −14.5 mV, dV_E35stop = 10.1 mV, V_½,∆6-46 = −15.8 mV, dV_∆6-46 = 10.6 mV).