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. 2015 Jul 8;5:11902. doi: 10.1038/srep11902

Figure 5. The optimal temperature for the MCDA assay.

Figure 5

The MCDA reactions were monitored by real-time measurement of turbidity and the corresponding curves of concentrations of DNA were marked in the Figure. The threshold value was 0.1 and the turbidity of >0.1 was considered to be positive. Eight kinetic graphs (AH) were obtained at different temperature (60–67 °C) with L. monocytogenes DNA at the level of 250 pg per reaction, and the graphs from B to F showed robust amplification.