Table 2. A comparison of oocytes retrieved from deer mice after injecting with PMSG using two different methods: GVBD, germinal vesicle breakdown.
Method | Dose (IU) | No. of mice treated | No. of COCs in total (per animal) | No. (%c) of oocytes at | No. (mean ± SD) of MII oocytes retrieved in total (per animal) | |
---|---|---|---|---|---|---|
GVBD | MII | |||||
1-PMSGa | 5 | 5 | 89 (17.8 ± 4.9) | 89 (100) | 56 (63) | 56 (11.2 ± 3.1) |
2-PMSGb | 15 | 5 | 75 (15 ± 4.2) | 75 (100) | 37 (49) | 37 (7.4 ± 2.7) |
aThe 1-PMSG method consists of one PMSG injections and COCs were isolated 56 h after the PMSG administration.
bThe 2-PMSG method includes a second administration of PMSG 24 h after the first one at the same dose, and COCs were isolated from ovaries 24 h after the 2nd PMSG administration.
cPercentage of the total number of COCs