Skip to main content
. 2015 Jul 15;5:11979. doi: 10.1038/srep11979

Figure 3. Sequence-dependent repression of AFP promoter activity by ZBTB20.

Figure 3

(a) Schematic demonstration of the WT and mutant AFP luciferase reporters starting from −837. The mutant nucleotides were shown in lower case. The HNF1 sites at AFP promoter were represented with open ovals. (b) The activity and ZBTB20-responsiveness of different mutant AFP reporters in HepG2 cells. The reporter plasmids were cotransfected into HepG2 cells with mock control vector (gray bar) or ZBTB20-expressing plasmids (black bar). RLU values were normalized to the activity of internal control RL-SV40. n = 3 experiments. *P < 0.05, **P < 0.01 vs mock control. (c) Sequence alignment of mouse AFP gene fragment −104/−86 with mouse albumin gene, and rat and human AFP genes.